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Western blot analysis of CHIA Antibody in mouse liver tissue lysates (35ug/lane)
Flow cytometric analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CHIA Antibody in mouse liver tissue lysates (35ug/lane)

CHIA Antibody

CATALOG NUMBER: 63-924

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This CHIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-47 amino acids from the N-terminal region of human CHIA.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
52 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CHIA
Additional Names:
Acidic mammalian chitinase, AMCase, Lung-specific protein TSA1902, CHIA
Protein Accession Number:
Q9BZP6
Protein Gi Number:
37999771
Ncbi Gene Id Number:
27159
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CHIA degrades chitin and chitotriose. May participate in the defense against nematodes and other pathogens. There are 3 named isoforms produced by alternative splicing. CHIA is induced via a T helper-2 (Th2)-specific, interleukin-13-mediated pathway in epithelial cells and macrophages. CHIA may be an important mediator of IL13-induced responses in Th2-dominated disorders such as asthma. CHIA hydrolysis N-acetyl-beta-D-glucosaminide 1,4-beta-linkages in chitin and chitodextrins.
Background Reference 1:
Wu, A.C., et al. J. Allergy Clin. Immunol. 125(3):754-757(2010)
Background Reference 2:
Ober, C., et al. Curr Opin Allergy Clin Immunol 9(5):401-408(2009)
Background Reference 3:
Seibold, M.A., et al. J. Biol. Chem. 284(29):19650-19658(2009)
Background Reference 4:
Hartl, D., et al. J. Immunol. 182(8):5098-5106(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-924

List Size:
400 ul

List Price:

$495.00

Datasheet
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