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Western blot analysis of extracts of various cell lines, using CHIT1 antibody (18-448) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human normal stomach using CHIT1 antibody (18-448) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using CHIT1 antibody (18-448) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

CHIT1 Antibody

CATALOG NUMBER: 18-448

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 217-466 of human CHIT1 (NP_003456.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
U-251MG
Positive Control 2:
293T
Positive Control 3:
Mouse spleen
Positive Control 4:
Mouse skeletal muscle
Predicted Molecular Weight:
Observed: 52kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CHIT1
Additional Names:
CHIT1, plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase, OTTHUMP00000217085, OTTHUMP00000217084, OTTHUMP00000217083, MGC125322, FLJ00314, chitotriosidase-1, chitinase-1, chitinase 1 (chitotriosidase), CHIT, CHI3, CHITD
Ncbi Gene Id Number:
1118
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-448

List Size:
100 uL

List Price:

$436.00

Datasheet
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