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Western blot analysis using CHK1 monoclonal antibody against NIH / 3T3 cell lysate.
Western blot analysis using CHK1 monoclonal antibody against NIH / 3T3 cell lysate.

CHK1 Antibody [2G11D5]

CATALOG NUMBER: 32-135

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified recombinant protein CHK1 expressed in E. Coli strain M15.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:Dilution 1:2,00 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein G affinity chromatography.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
CHK1 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CHEK1
Additional Names:
CHK1
Protein Accession Number:
NP_001107593.1
Protein Gi Number:
166295194
Ncbi Gene Id Number:
1111
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Active recombinant full length human CHK1 containing an Nterminal GST tag. This protein has an apparent molecular weight of ~82 kDa. CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia.
Background Reference 1:
EMBO J. 1998. 16: 545-554.
Background Reference 2:
Science 1998. 282: 1893-1897.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-135

List Size:
0.1 mg

List Price:

$370.00

Datasheet
Shipping Info

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