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Immunohistochemical analysis of paraffin-embedded H. stomach section using CHML Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Hela cells using CHML Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded R. cerebellum section using CHML Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded M. cerebellum section using CHML Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysate from HT-1080 cell line, using CHML Antibody at 1:1000.
Immunohistochemical analysis of paraffin-embedded H. stomach section using CHML Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

CHML Antibody

CATALOG NUMBER: 61-049

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CHML antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 624-656 amino acids from the C-terminal region of human CHML.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For FACS starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
74 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CHML
Additional Names:
Rab proteins geranylgeranyltransferase component A 2, Choroideremia-like protein, Rab escort protein 2, REP-2, CHML, REP2
Protein Accession Number:
P26374
Protein Gi Number:
47117837
Ncbi Gene Id Number:
1122
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family.
Background Reference 1:
Cremers F.P.M.,et al.Hum. Mol. Genet. 1:71-75(1992).
Background Reference 2:
Kasper G.,et al.Gene 295:27-32(2002).
Background Reference 3:
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Background Reference 4:
Gregory S.G.,et al.Nature 441:315-321(2006).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-049

List Size:
400 ul

List Price:

$469.00

Out of stock

Datasheet
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