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Western blot analysis of extracts of various cell lines, using CHMP1B antibody (23-474) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse kidney using CHMP1B antibody (23-474) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using CHMP1B antibody (23-474) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using CHMP1B antibody (23-474) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using CHMP1B antibody (23-474) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

CHMP1B Antibody

CATALOG NUMBER: 23-474

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human CHMP1B (NP_065145.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
NCI-H460
Positive Control 2:
MCF7
Positive Control 3:
SKOV3
Positive Control 4:
U-251MG
Positive Control 5:
Mouse liver
Positive Control 6:
Mouse spleen
Predicted Molecular Weight:
Observed: 30kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CHMP1B
Additional Names:
C10orf2, C18-ORF2, C18orf2, CHMP1.5, Vps46-2, Vps46B, hVps46-2, charged multivesicular body protein 1b, chromatin modifying protein 1B, chromatin-modifying protein 1b, vacuolar protein sorting 46-2, vacuolar protein sorting-associated protein 46-2
Ncbi Gene Id Number:
57132
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-474

List Size:
100 uL

List Price:

$436.00

Datasheet
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