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Western blot analysis of extracts of various cell lines, using CHP1 antibody (16-234) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using CHP1 antibody (16-234) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

CHP1 Antibody

CATALOG NUMBER: 16-234

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human CHP1 (NP_009167.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
LO2
Positive Control 2:
293T
Positive Control 3:
HeLa
Positive Control 4:
Mouse brain
Positive Control 5:
Mouse heart
Positive Control 6:
Mouse kidney
Predicted Molecular Weight:
Observed: 20kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CHP1
Additional Names:
CHP, SLC9A1BP, SPAX9, Sid470p, p22, p24, calcineurin B homologous protein 1, EF-hand calcium-binding domain-containing protein p22, SLC9A1 binding protein, calcineurin B homolog, calcineurin B-like protein, calcineurin homologous protein, calcium binding protein P22, calcium-binding protein CHP, calcium-binding protein p22
Ncbi Gene Id Number:
11261
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a phosphoprotein that binds to the Na+/H+ exchanger NHE1. This protein serves as an essential cofactor which supports the physiological activity of NHE family members and may play a role in the mitogenic regulation of NHE1. The protein shares similarity with calcineurin B and calmodulin and it is also known to be an endogenous inhibitor of calcineurin activity.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-234

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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