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Western blot analysis of CHPF Antibody in MDA-MB435, MCF-7, HepG2, A375 cell line and mouse testis tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with CHPF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CHPF Antibody in MDA-MB435, MCF-7, HepG2, A375 cell line and mouse testis tissue lysates (35ug/lane)

CHPF Antibody

CATALOG NUMBER: 63-759

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This CHPF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 327-354 amino acids from the Central region of human CHPF.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
85 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CHPF
Additional Names:
Chondroitin sulfate synthase 2, Chondroitin glucuronyltransferase 2, Chondroitin-polymerizing factor, ChPF, Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II, N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase II, N-acetylgalactosaminyltransferase 2, CHPF, CSS2
Protein Accession Number:
Q8IZ52
Protein Gi Number:
311033361
Ncbi Gene Id Number:
79586
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CHPF is a protein that has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer.
Background Reference 1:
Matsuoka,S., et.al., Science 316 (5828), 1160-1166 (2007)
Background Reference 2:
Colland,F., et.al., Genome Res. 14 (7), 1324-1332 (2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-759

List Size:
400 ul

List Price:

$495.00

Datasheet
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