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Western Blot at 1:2000 dilution Lane 1: HT-29 whole cell lysate Lane 2: SW620 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: HT-29 whole cell lysate Lane 2: SW620 whole cell lysate Lysates/proteins at 20 ug per lane.

CHRM3 Antibody

CATALOG NUMBER: 61-333

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CHRM3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 382-416 amino acids from human CHRM3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
66 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CHRM3
Additional Names:
Muscarinic acetylcholine receptor M3, CHRM3
Protein Accession Number:
P20309
Protein Gi Number:
113125
Ncbi Gene Id Number:
1131
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Background Reference 1:
Peralta E.G.,et al.EMBO J. 6:3923-3929(1987).
Background Reference 2:
Bonner T.I.,et al.Neuron 1:403-410(1988).
Background Reference 3:
Kitano T.,et al.Mol. Biol. Evol. 21:936-944(2004).
Background Reference 4:
Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-333

List Size:
200 ul

List Price:

$495.00

Datasheet
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