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42-389 (1ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-389 (3.75ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-389 (1ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

CHRNA5 Antibody

CATALOG NUMBER: 42-389

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-HVDRYFTQKEET
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in lysates of cell line KELLY (calculated MW of 53.1kDa according to NP_000736.2). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon (submucosal plexus). Recommended concentration: 3.75ug/ml.
Positive Control 1:
KELLY (Human neuroblastoma cell line) Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CHRNA5
Additional Names:
CHRNA5, cholinergic receptor, nicotinic, alpha 5, LNCR2, Cholinergic receptor, neuronal nicotinic, alpha polypeptide-5, neuronal acetylcholine receptor subunit alpha-5, neuronal nicotinic acetylcholine receptor, alpha5 subunit
Protein Accession Number:
NP_000736.2
Protein Gi Number:
33589827
Ncbi Gene Id Number:
1138

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-389

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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