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43-140 (5ug/ml) staining of paraffin embedded Rat Cerebral Cortex (A) and Rat Hippocampus (B), CRY-staining with DAPI nuclear counter stain in blue. Antigen retrieval with citrate buffer pH 6 at 95-98C.
43-140 (3ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-140 (5ug/ml) staining of paraffin embedded Rat Cerebral Cortex (A) and Rat Hippocampus (B), CRY-staining with DAPI nuclear counter stain in blue. Antigen retrieval with citrate buffer pH 6 at 95-98C.

CHRNA7 Antibody

CATALOG NUMBER: 43-140

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: KRPGEDKVRPACQHKQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Immunohistochemistry:In paraffin embedded Rat Cerebral Cortex and Rat Hippocampus shows membranous staining in neural cells. Recommended concentration: 5-10ug/ml. Paraffin embedded Human Brain (Cortex). Recommended concentration: 3-6ug/ml.
Specificity:
This antibody also reacts with CHRNA7-FAM7A fusion isoform 1 (NP_647536.1) and isoform 2 (NP_683709.1).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CHRNA7
Additional Names:
CHRNA7, cholinergic receptor, nicotinic, alpha 7, NACHRA7, a7 nicotinic acetylcholine receptor, alpha 7 neuronal nicotinic acetylcholine receptor, alpha-7 nicotinic cholinergic receptor subunit, alpha7 nicotinic receptor subunit, cholinergic receptor, nicotinic, alpha polypeptide 7, neuronal acetylcholine receptor protein, alpha-7 chain, neuronal nicotinic acetylcholine receptor alpha-7 subunit, nicotinic acetylcholine alpha-7 subunit, nicotinic acetylcholine receptor alpha7 subunit, nicotinic cholinergic receptor alpha 7
Protein Accession Number:
NP_000737.1
Protein Gi Number:
4502831
Ncbi Gene Id Number:
1139
Background Reference 1:
Ren K, King MA, Liu J, Siemann J, Altman M, Meyers C, Hughes JA, Meyer EMThe alpha7 nicotinic receptor agonist 4OH-GTS-21 protects axotomized septohippocampal cholinergic neurons in wild type but not amyloid-overexpressing transgenic mice

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-140

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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