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Flow cytometric analysis of Jurkat cells using CHRNA9 Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysate from Daudi cell line, using CHRNA9 Antibody at 1:1000.
Flow cytometric analysis of Jurkat cells using CHRNA9 Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

CHRNA9 Antibody

CATALOG NUMBER: 61-055

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CHRNA9 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 8-42 amino acids from the N-terminal region of human CHRNA9.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For FACS starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
55 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CHRNA9
Additional Names:
Neuronal acetylcholine receptor subunit alpha-9, Nicotinic acetylcholine receptor subunit alpha-9, NACHR alpha-9, CHRNA9, NACHRA9
Protein Accession Number:
Q9UGM1
Protein Gi Number:
308153406
Ncbi Gene Id Number:
55584
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. May also regulate keratinocyte adhesion.
Background Reference 1:
Sgard F.,et al.Mol. Pharmacol. 61:150-159(2002).
Background Reference 2:
Lustig L.R.,et al.Cytogenet. Genome Res. 98:154-159(2002).
Background Reference 3:
Hillier L.W.,et al.Nature 434:724-731(2005).
Background Reference 4:
Nguyen V.T.,et al.Am. J. Pathol. 157:1377-1391(2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-055

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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