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46-825 (2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-825 (2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

CHRNB4 Antibody

CATALOG NUMBER: 46-825

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-DYRLTWNSSRYEGVN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 40kDa band in human testis lysates after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4kDa according to NP_000741.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 2.5ug/ml.
Specificity:
The immunizing peptide represents part of the first extracellular domain (aa22-236).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CHRNB4
Additional Names:
CHRNB4 , cholinergic receptor, nicotinicN: beta 4 , Cholinergic receptor, neuronal nicotinic, beta polypeptide-4, cholinergic receptor, nicotinic, beta polypeptide 4, neuronal nicotinic receptor beta 4 subunit
Protein Accession Number:
NP_000741.1
Protein Gi Number:
4502837
Ncbi Gene Id Number:
1143
Background Reference 1:
Liang Y, Salas R, Marubio L, Bercovich D, De Biasi M, Beaudet AL, Dani JA. Functional polymorphisms in the human beta4 subunit of nicotinic acetylcholine receptors. Neurogenetics. 2005 Feb;6(1):37-44. Epub 2004 Nov 25.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-825

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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