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45-039 (0.1ug/ml) staining of isoform 1+ 2 Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-039 (3.75ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-039 (3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-039 (2ug/ml) staining of isoform 1 Human Brain (A) and isoform 1+2 (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-039 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
45-039 (0.1ug/ml) staining of isoform 1+ 2 Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Chromogranin A Antibody

CATALOG NUMBER: 45-039

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-QGAKERAHQQKKH
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 50kDa band observed in Human Cerebellum and Kidney lysates. An additional band at 35kDa was observed in some lysates (calculated MW of 50.7 kDa according to NP_001266.1 isoform 1 and 34.3kDa according to NP_001288619.1 isoform 2). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Pancreas and Small Intestine. Recommended concentration: 3.75ug/ml.Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CHGA
Additional Names:
chromogranin A precursor, chromogranin A (parathyroid secretory protein 1) , CHGA, CGA , parathyroid secretory protein 1, Vasostatin, Pancreastatin, Parastatin, Pituitary secretory protein I, SP1
Protein Accession Number:
NP_001266.1,NP_001288619.1
Protein Gi Number:
4502805
Ncbi Gene Id Number:
1113
Background Reference 1:
Li L, Yuan YZ, Lu J, Xia L, Zhu Y, Zhang YP, Qiao MM. Treatment of pancreatic carcinoma by adenoviral mediated gene transfer of vasostatin in mice. Gut. 2006 Feb;55(2):259-65. Epub 2005 Nov 15.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-039

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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