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Western blot analysis of hromogranin-C Antibody in Ramos cell line lysates (35ug/lane)
Chromogranin-C Antibody IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Chromogranin-C Antibody for immunohistochemistry.
Western blot analysis of hromogranin-C Antibody in Ramos cell line lysates (35ug/lane)

Chromogranin-C Antibody

CATALOG NUMBER: 62-471

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Pig
Immunogen:
This Chromogranin-C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-274 amino acids from the Central region of human Chromogranin-C.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
71 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SCG2
Additional Names:
Secretogranin-2, Chromogranin-C, Secretogranin II, SgII, Secretoneurin, SN, SCG2, CHGC
Protein Accession Number:
P13521
Protein Gi Number:
143811457
Ncbi Gene Id Number:
7857
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Chromogranin-C is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Studies in rodents suggest that the full-length protein, secretogranin II, is involved in the packaging or sorting of peptide hormones and neuropeptides into secretory vesicles. The full-length protein is cleaved to produce the active peptide secretoneurin, which exerts chemotaxic effects on specific cell types, and EM66, whose function is unknown.
Background Reference 1:
Li,L., et.al., Cell Death Differ. 15 (5), 879-888 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-471

List Size:
400 ul

List Price:

$495.00

Datasheet
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