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Antibody used in IHC on Human Muscle.
Antibody used in WB on Human Jurkat 1.25 ug/ml.
Antibody used in IHC on Human Muscle.

CHST1 Antibody

CATALOG NUMBER: 30-071

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHST1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
CHST1 antibody can be used for detection of CHST1 by ELISA at 1:1562500. CHST1 antibody can be used for detection of CHST1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
47 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store CHST1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
CHST1
Additional Names:
CHST1, C6ST, KS6ST, KSGal6ST, KSST, GST-1
Protein Accession Number:
NP_003645
Protein Gi Number:
4502841
Ncbi Gene Id Number:
8534
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CHST1 catalyzes the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. It has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. It may function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. It participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation.
Background Reference 1:
Yamada, T., Biochem. J. 384 (PT 3), 567-575 (2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-071

List Size:
100 ul

List Price:

$473.00

Datasheet
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