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43-473 (1ug/ml) staining of HeLa nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-473 (1ug/ml) staining of HeLa nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CHUK Antibody

CATALOG NUMBER: 43-473

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: EHDHSLSCVVTPQD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 84.6kDa according to NP_001269.3). Recommended concentration: 1-3ug/ml.
Predicted Molecular Weight:
Approx 80kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CHUK
Additional Names:
CHUK, conserved helix-loop-helix ubiquitous kinase, IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16, I-kappa-B kinase 1, I-kappa-B kinase-alpha, IKK-a kinase, IkB kinase alpha subunit, Nuclear factor NFkappaB inhibitor kinase alpha, TCF-16, inhibitor of nuclear factor kappa-B kinase subunit alpha, transcription factor 16
Protein Accession Number:
NP_001269.3
Protein Gi Number:
2327068
Ncbi Gene Id Number:
1147
Background Reference 1:
Karin M.How NF-kappaB is activated: the role of the IkappaB kinase (IKK) complex.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-473

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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