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Western blot analysis using CIB1 monoclonal antibody against truncated CIB1 recombinant protein and A431 cell lysate.
Immunohistochemical analysis of paraffin - embedded human glioma tissue showing membrane location using CIB1 antibody with DAB staining.
Western blot analysis using CIB1 monoclonal antibody against truncated CIB1 recombinant protein and A431 cell lysate.

CIB1 Antibody [5A1F5E12 / 5A1H7E12]

CATALOG NUMBER: 32-136

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant CIB1 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
CIB1 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CIB1
Additional Names:
CIB, CIBP, KIP1, PRKDCIP, SIP2-28
Protein Accession Number:
NP_001264693.1
Protein Gi Number:
480540325
Ncbi Gene Id Number:
10519
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CIB1(also designated calcium and integrin binding 1 or calmyrin),with 191-amino acid protein(about 21 kDa), belongs to the calcium-binding protein family.CIB1 is known to interact with DNA-dependent protein kinase and may play a role in kinase-phosphatase regulation of DNA end joining.CIB1 is an EF-hand-containing protein that binds multiple effector proteins, including the platelet alpha(IIb)beta(3) integrin and several serine/threonine kinases and potentially modulates their function.CIB1 regulates platelet aggregation in hemostasis through a specific interaction with the alpha(IIb) cytoplasmic domain of platelet integrin alpha(IIb)beta(3). CIB1 is also ubiquitously expressed activating and inhibiting protein ligand of the InsP3R.
Background Reference 1:
Holly R. Gentry,Alex U. Singer, Laurie Betts. J. Biol. Chem., Mar 2005; 280: 8407 - 8415.
Background Reference 2:
Carl White, Jun Yang, Mervyn J. Monteiro. J. Biol. Chem., Jul 2006; 281: 20825 – 20833.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-136

List Size:
0.1 mg

List Price:

$432.00

Datasheet
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