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Western blot analysis of CIDE-B in mouse small intestine tissue lysate with CIDE-B antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunohistochemistry of CIDE-B in human small intestine tissue with CIDE-B antibody at 5 μg/mL.
Immunofluorescence of CIDE-B in Human Small Intestine cells with CIDE-B antibody at 20 μg/mL.
Western blot analysis of CIDE-B in mouse small intestine tissue lysate with CIDE-B antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Trial Size

CIDE-B Antibody

CATALOG NUMBER: 2321

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (86%)
Immunogen:
CIDE-B antibody was raised against a peptide corresponding to 14 amino acids near the center of human CIDE-B.
The immunogen is located within amino acids 100 - 150 of CIDE-B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
CIDE-B antibody can be used for detection of CIDE-B by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
CIDE-B antibody has no cross activity to CIDE-A.
Positive Control 1:
Cat. No. 1408 - Mouse Small Intestine Tissue Lysate
Positive Control 2:
Cat. No. 1308 - Human Small Intestine Tissue Lysate
Positive Control 3:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Purification:
CIDE-B Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CIDE-B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
CIDE-B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CIDEB
Additional Names:
CIDE-B Antibody: CIDE-B, AI790179, 1110030C18Rik, Cell death activator CIDE-B, Cell death-inducing DFFA-like effector B
Protein Accession Number:
AF218586
Protein Gi Number:
6746626
Ncbi Gene Id Number:
27141
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CIDE-B Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-B induces apoptosis, which is inhibited by DFF45. CIDE-B is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-B is expressed mainly in liver and at lower levels in spleen, kidney, peripheral blood lymphocytes and bone marrow.
Background Reference 1:
Inohara N, Koseki T, Chen S,et al. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J. 1998; 17:2526-33.
Background Reference 2:
Inohara N, Koseki T, Chen S, et al. Identification of regulatory and catalytic domains in the apoptosis nuclease DFF40/CAD. J. Biol. Chem.1999; 274:270-4.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2321

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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