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Western blot analysis of CIKS expression in human lung (lane A) and placenta (lane B) tissue lysates with CIKS antibody at 1 μg/ml.
Immunohistochemistry of CIKS in human lung tissue with CIKS antibody at 5 μg/mL.
Immunofluorescence of CIKS in human lung tissue with CIKS antibody at 20 μg/mL.
Immunofluorescence of CIKS in A549 cells with CIKS antibody at 20 μg/mL.<br><br>Green: CIKS Antibody (2501) <br> Blue: DAPI staining
Western blot analysis of CIKS expression in human lung (lane A) and placenta (lane B) tissue lysates with CIKS antibody at 1 μg/ml.
Trial Size

CIKS Antibody

CATALOG NUMBER: 2501

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
CIKS antibody was raised against a 14 amino acid peptide near the amino terminus of human CIKS.
The immunogen is located within the first 50 amino acids of CIKS.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
CIKS antibody can be used for detection of CIKS by Western blot at 1 μg/mL. A band at approximately 63 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1309 - Human Placenta Tissue Lysate
Positive Control 2:
Cat. No. 1302 - Human Lung Tissue Lysate
Positive Control 3:
Cat. No. 10-101 - Human Lung Tissue Slide
Predicted Molecular Weight:
63 kDa
Purification:
CIKS Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CIKS Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
CIKS antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRAF3IP2
Additional Names:
CIKS Antibody: ACT1, CIKS, C6orf2, C6orf4, C6orf5, C6orf6, CANDF8, PSORS13, Adapter protein CIKS, Nuclear factor NF-kappa-B activator 1, ACT1
Protein Accession Number:
O43734
Protein Gi Number:
10242289
Ncbi Gene Id Number:
10758
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CIKS Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB associates with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB is phosphorylated by IκB kinase (IKK) complex that contains IKKα, IKKβ, and IKKγ. A novel molecule that associates with and activates IKK was recently identified and designated CIKS (for connection to IKK and SAPK/JNK) and Act1 (for NF-κB activator 1). CIKS directly interacts with IKKγ. CIKS/Act1 also activates activating transcription factor (ATF) and activator protein 1 (AP-1) through Jun kinase (JNK). These results indicate that CIKS/Act1 is involved in the inflammation and stress responses. CIKS/Act1 is ubiquitously expressed in human tissues.
Background Reference 1:
Leonardi A, Chariot A, Claudio E, Cunningham K, Siebenlist U. CIKS, a connection to Iκ B kinase and stress-activated protein kinase. Proc Natl Acad Sci USA. 2000;97(19):10494-9.
Background Reference 2:
Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D, Haag M, Stark GR. Act1, an NF-κ B activating protein. Proc Natl Acad Sci USA. 2000;97(19):10489-93.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2501

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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