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45-402 (0.03ug/ml) staining of rat spinal cord lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-402 (0.03ug/ml) staining of rat spinal cord lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CILP Antibody

CATALOG NUMBER: 45-402

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Dog
Immunogen:
The immunogen for this antibody is: C-NYRRTDHEDPRVK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in rat spinal cord lysates (calculated MW of 133kDa according to human NP_003604.2 and of 132kDa according to Rat NP_001101631.1). Recommended concentration: 0.1-0.3ug/ml.
Positive Control 1:
Rat Spinal Cord Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CILP
Additional Names:
CILP, cartilage intermediate layer protein, nucleotide pyrophosphohydrolase , HGNC:1980, HsT18872 , cartilage intermediate layer protein
Protein Accession Number:
NP_003604.2
Protein Gi Number:
51944962
Ncbi Gene Id Number:
8483
Background Reference 1:
Seki S, Kawaguchi Y, Chiba K, Mikami Y, Kizawa H, Oya T, Mio F, Mori M, Miyamoto Y, Masuda I, Tsunoda T, Kamata M, Kubo T, Toyama Y, Kimura T, Nakamura Y, Ikegawa S. A functional SNP in CILP, encoding cartilage intermediate layer protein, is associated with susceptibility to lumbar disc disease. Nat Genet. 2005 Jun;37(6):607-12. Epub 2005 May 1.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-402

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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