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42-632 (0.05ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-632 (0.05ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CIRBP Antibody

CATALOG NUMBER: 42-632

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QAGKSSDNRSR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 18kDa band observed in Mouse and Rat Testis lysates (calculated MW of 18.6kDa according to Mouse NP_031731.1 and Rat NP_112409.2). Recommended concentration: 0.05-0.2ug/ml.An additional band of unknown identity was also consistently observed at 23kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Positive Control 1:
Cat. No. 1416 - Mouse Testis Tissue Lysate
Positive Control 2:
Cat. No. 1476 - Rat Testis Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CIRBP
Additional Names:
CIRBP, cold inducible RNA binding protein, CIRP, Cold-inducible RNA-binding protein, cold inducible RNA-binding protein, glycine-rich RNA binding protein
Protein Accession Number:
NP_001271.1
Protein Gi Number:
4502847
Ncbi Gene Id Number:
1153

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-632

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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