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Western blot analysis of CIRBP Antibody in Y79 cell line lysates (35ug/lane)
CIRBP Antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of CIRBP using rabbit polyclonal CIRBP Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CIRBP gene.
Western blot analysis of CIRBP Antibody in Y79 cell line lysates (35ug/lane)

CIRBP Antibody

CATALOG NUMBER: 63-800

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Hamster
Immunogen:
This CIRBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-172 amino acids from the C-terminal region of human CIRBP.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
19 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CIRBP
Additional Names:
Cold-inducible RNA-binding protein, A18 hnRNP, Glycine-rich RNA-binding protein CIRP, CIRBP, A18HNRNP, CIRP
Protein Accession Number:
Q14011
Protein Gi Number:
5921786
Ncbi Gene Id Number:
1153
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CIRBP is cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. It acts as a translational activator and seems to play an essential role in cold-induced suppression of cell proliferation. It binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. This protein acts as a translational repressor (By similarity) and promotes assembly of stress granules (SGs), when overexpressed.
Background Reference 1:
Colinge J., et.al., Submitted (OCT-2008) to UniProtKB.
Background Reference 2:
Yang R.,et.al., Nucleic Acids Res. 34:1224-1236(2006).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-800

List Size:
400 ul

List Price:

$495.00

Datasheet
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