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Western blot analysis of CISD2 in rat brain tissue lysate with CISD2 antibody at (A) 1 and (B) 2 μg/m
Immunohistochemistry of CISD2 in rat brain tissue with CISD2 antibody at 2.5 μg/ml.
Immunofluorescence of CISD2 in rat brain tissue with CISD2 antibody at 20 μg/ml.
Western blot analysis of CISD2 in rat brain tissue lysate with CISD2 antibody at (A) 1 and (B) 2 μg/m
Trial Size

CISD2 Antibody

CATALOG NUMBER: 7301

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
CISD2 antibody was raised against a 15 amino acid peptide near the center of human CISD2.
The immunogen is located within amino acids 40 - 90 of CISD2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
CISD2 antibody was raised against a 15 amino acid peptide near the center of human CISD2.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 15 kDa
Observed: 15 kDa
Purification:
CISD2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CISD2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CISD2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
CISD2
Additional Names:
CISD2 Antibody: ERIS, WFS2, ZCD2, NAF-1, Miner1, CDGSH2, ERIS, CDGSH iron-sulfur domain-containing protein 2, Endoplasmic reticulum intermembrane small protein
Protein Accession Number:
NP_001008389
Protein Gi Number:
56605994
Ncbi Gene Id Number:
493856
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CISD2 Antibody: Defects in the CISD2 (CDGSH iron sulfur domain 2) gene are a cause of the neurodegenerative disorder Wolfram syndrome 2. CISD2 is a zinc finger protein that localizes to the endoplasmic reticulum and mitochondria and binds an iron/sulfur cluster. CISD2 interacts with Bcl-2 and can be displaced by the BH3-only protein BIK and contributes to the regulation of BIK-initiated autophagy. CISD2 deficiency in mice causes mitochondrial breakdown accompanied by autophagic cell death as well as the development of premature aging phenotype.
Background Reference 1:
Amr S, Heisey C, Zhang M, et al. A homozygous mutation in a novel zinc-finger protein, ERIS, is responsible for Wolfram syndrome 2. Am. J. Hum. Genet. 2007; 81:673-83.
Background Reference 2:
Chang NC, Nguyen M, Germain M, et al. Antagonism of Beclin 1-dependent autophagy by BCL-2 at the endoplasmic reticulum requires NAF-1. EMBO J. 2010; 29:606-18.
Background Reference 3:
Chen YF, Kao CH, Chen YT, et al. Cisd2 deficiency drives premature aging and causes mitochondria-mediated defects in mice.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7301

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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