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Western blot analysis of extracts from HT-29 cells treated with heat shock using CK-1alpha (Phospho-Tyr294) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using CK-1alpha (Phospho-Tyr294) antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from HT-29 cells treated with heat shock using CK-1alpha (Phospho-Tyr294) Antibody. The lane on the right is treated with the antigen-specific peptide.

CK-1alpha (phospho Tyr294) Antibody

CATALOG NUMBER: 79-842

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
CK-1alpha (phospho Tyr294) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y (p)-T-F) derived from Human CK-1alpha.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
The antibody detects endogenous levels of CK-1alpha only when phosphorylated at tyrosine 294.
Predicted Molecular Weight:
37 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CSNK1A1, CSNK1A1L
Additional Names:
KC1A, CKI-alpha, CSNK1A1, alpha isoform, Casein kinase I
Protein Accession Number:
NP_001883.4
Protein Gi Number:
68303575
Ncbi Gene Id Number:
1452;122011
Background:
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
Background Reference 1:
Tapia C., FEBS Lett. 349:307-312(1994).
Background Reference 2:
Fish K.J., J. Biol. Chem. 270:14875-14883(1995).
Background Reference 3:
Halleck A., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-842

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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