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Western blot analysis using CK1 monoclonal antibody against truncated CK1 recombinant protein.
Immunohistochemical analysis of paraffin - embedded human normal liver tissue (top) and human colon carcinoma (bottom) showing membrane location using CK1 antibody with DAB staining.
Western blot analysis using CK1 monoclonal antibody against truncated CK1 recombinant protein.

CK1 Antibody [3C10F7 / 3C10G5 / PMC01 / 4D12B3]

CATALOG NUMBER: 32-137

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant CK1 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
CK1 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CSNK1A1
Additional Names:
CK1, CKI-alpha, CK1a, CKIa, HLCDGP1, PRO2975, HEL-S-77p
Protein Accession Number:
P48729
Protein Gi Number:
31077177
Ncbi Gene Id Number:
1452
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CK1 (also designated Cytokeratin 1 or KRT1 ),with 644-amino acid protein (about 70 kDa), is a member of the keratin family. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells.CK1 consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. CK1 is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.
Background Reference 1:
Ricardo G. Espinola, Robin A. Pixley, Berhane Ghebrehiwet. Blood (ASH Annual Meeting Abstracts), Nov 2005; 106: 2666.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-137

List Size:
0.1 mL

List Price:

$408.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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