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Western Blot at 1:2000 dilution Lane 1: C6 whole cell lysates Lane 2: MOLT-4 whole cell lysates Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma
Western Blot at 1:2000 dilution Lane 1: C6 whole cell lysates Lane 2: MOLT-4 whole cell lysates Lysates/proteins at 20 ug per lane.

CKII alpha Antibody

CATALOG NUMBER: 63-424

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Rabbit, Xenopus
Immunogen:
This CKII alpha (CSNK2A1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-269 amino acids from the Central region of human CKII alpha (CSNK2A1).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CSNK2A1
Additional Names:
Casein kinase II subunit alpha, CK II alpha, CSNK2A1, CK2A1
Protein Accession Number:
P68400
Protein Gi Number:
55977123
Ncbi Gene Id Number:
1457
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation.
Background Reference 1:
Miyata, Y., et al., Mol. Cell. Biol. 24(9):4065-4074 (2004).
Background Reference 2:
Loizou, J.I., et al., Cell 117(1):17-28 (2004).
Background Reference 3:
Filhol, O., et al., EMBO Rep. 5(4):351-355 (2004).
Background Reference 4:
Kulartz, M., et al., Biochem. Biophys. Res. Commun. 315(4):1011-1017 (2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-424

List Size:
400 ul

List Price:

$495.00

Datasheet
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