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Antibody is used in Western blot to detect CKM in C6 cell lysate.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Flow cytometric analysis of HepG2 cells using Creatine Kinase MB (CKM) Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Antibody is used in Western blot to detect CKM in C6 cell lysate.

CKM Antibody

CATALOG NUMBER: 62-637

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Pig, Rb
Immunogen:
This CKM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-313 amino acids from the C-terminal region of human CKM.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CKM
Additional Names:
Creatine kinase M-type, Creatine kinase M chain, M-CK, Creatine kinase M-type, N-terminally processed, CKM, CKMM
Protein Accession Number:
P06732
Protein Gi Number:
125305
Ncbi Gene Id Number:
1158
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. The CKM isoform, predominant in skeletal muscle and heart tissue, is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-637

List Size:
400 ul

List Price:

$495.00

Datasheet
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