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IHC testing of FFPE human pancreas with Clathrin Light Chain antibody (clone CLC/1421). Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free Clathrin Light Chain Antibody (clone CLC/1421). Confirmation of Integrity and Purity of the Antibody.
IHC testing of FFPE human pancreas with Clathrin Light Chain antibody (clone CLC/1421). Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min.

Clatherin Light Chain Antibody [CLC/1421]

CATALOG NUMBER: 33-666

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
A recombinant N-terminal protein fragment from human Clathrin light chain was used as the immunogen for the Clathrin Light Chain antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
WB: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
Optimal dilution of the Clathrin Light Chain antibody should be determined by the researcher.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2b, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CLTA, CLTB
Ncbi Gene Id Number:
12111212
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Recognizes proteins of 31-44kDa, which are identified as Clathrin Light Chains (both A & B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60% homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-666

List Size:
100 ug

List Price:

$626.00

Datasheet
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