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Western blot analysis of extracts from COLO cells treated with EGF using Claudin 3 (Phospho-Tyr219) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from COLO cells treated with EGF using Claudin 3 (Phospho-Tyr219) Antibody. The lane on the right is treated with the antigen-specific peptide.

Claudin 3 (phospho Tyr219) Antibody

CATALOG NUMBER: 79-901

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Claudin 3 (phospho Tyr219) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 219 (R-K-D-Y (p)-V) derived from Human Claudin 3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous levels of Claudin 3 only when phosphorylated at tyrosine 219.
Predicted Molecular Weight:
28 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CLDN3
Additional Names:
CLD3, CLDN3, HRVP1, CPE-R 2, CPE-receptor 2
Protein Accession Number:
NP_001297.1
Protein Gi Number:
6685273
Ncbi Gene Id Number:
1365
Background:
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands.
Background Reference 1:
Peacock R.E., Genomics 46:443-449(1997).
Background Reference 2:
Katahira J., J. Biol. Chem. 272:26652-26658(1997).
Background Reference 3:
The MGC Project Team; Genome Res. 14:2121-2127(2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-901

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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