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45-405 (1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-405 (1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CLCA1 Antibody

CATALOG NUMBER: 45-405

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-TVTSKTNKDTSK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Experiments gave bands at approx 80kDa and 30kDa in Human Duodenum lysates. These bands correspond to earlier findings in literature with different antibodies (Gruber et al, Genomics. 1998 Dec 1;54(2):200-14.). This protein has a calculated MW of 100kDa according to NP_001276.1. Recommended concentration: 1-3ug/ml.
Specificity:
No cross-reactivity expected with CLCA2 and 3.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CLCA1
Additional Names:
CLCA1, chloride channel, calcium activated, family member 1 , HGNC:2015, CaCC, calcium activated chloride channel 1
Protein Accession Number:
NP_001276.1
Protein Gi Number:
4502865
Ncbi Gene Id Number:
1179
Background Reference 1:
Malerba G, Pignatti PF. A review of asthma genetics: gene expression studies and recent candidates. J Appl Genet. 2005;46(1):93-104. Review.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-405

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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