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Antibody used in WB on Human kidney at 0.2-1 ug/ml.
Antibody used in WB on Human kidney at 0.2-1 ug/ml.

CLCNKB Antibody

CATALOG NUMBER: 28-218

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CLCNKB.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
CLCNKB antibody can be used for detection of CLCNKB by ELISA at 1:312500. CLCNKB antibody can be used for detection of CLCNKB by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Predicted Molecular Weight:
76 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store CLCNKB antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
CLCNKB
Additional Names:
CLCNKB, CLCKB, ClC-K2, ClC-Kb
Protein Accession Number:
NP_000076
Protein Gi Number:
155969705
Ncbi Gene Id Number:
1188
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Chloride channel Kb (CLCNKB) is a member of the CLC family of voltage-gated chloride channels, which comprises at least 9 mammalian chloride channels. Each is believed to have 12 transmembrane domains and intracellular N and C termini. Mutations in CLCNKB result in the autosomal recessive Type III Bartter Syndrome. CLCNKA and CLCNKB are closely related (94% sequence identity), tightly linked (separated by 11 kb of genomic sequence) and are both expressed in mammalian kidney.
Background Reference 1:
Gorgojo, J.J., et al., (2006) Horm. Res. 65 (2), 62-68.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-218

List Size:
100 ul

List Price:

$519.00

Datasheet
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