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Antibody used in IHC on Human Liver.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in IHC on Human Liver.

CLDN1 Antibody

CATALOG NUMBER: 27-907

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CLDN1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
CLDN1 antibody can be used for detection of CLDN1 by ELISA at 1:312500. CLDN1 antibody can be used for detection of CLDN1 by western blot at 1.0-5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
23 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store CLDN1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
CLDN1
Additional Names:
CLDN1, CLD1, SEMP1, ILVASC
Protein Accession Number:
NP_066924
Protein Gi Number:
10863887
Ncbi Gene Id Number:
9076
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. This protein, a member of the claudin family, is an integral membrane protein and a component of tight junction strands.
Background Reference 1:
Coyne, C.B., et al., (2003) Am. J. Physiol. Lung Cell Mol. Physiol. 285 (5), L1166-L1178.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-907

List Size:
100 ul

List Price:

$519.00

Datasheet
Shipping Info

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