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Western Blot at 1:1000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of CLDN2 using rabbit polyclonal CLDN2 Antibody (Y224) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLDN2 gene.
immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.

CLDN2 Antibody

CATALOG NUMBER: 56-552

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CLDN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-227 amino acids from the C-terminal region of human CLDN2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
25 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CLDN2
Additional Names:
Claudin-2, SP82, CLDN2
Protein Accession Number:
P57739
Protein Gi Number:
12229749
Ncbi Gene Id Number:
9075
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene product belongs to the claudin protein familywhose members have been identified as major integral membraneproteins localized exclusively at tight junctions. Claudins areexpressed in an organ-specific manner and regulate tissue-specificphysiologic properties of tight junctions. This protein isexpressed in the intestine. Alternatively spliced transcriptvariants with different 5' untranslated region have been found forthis gene.
Background Reference 1:
Smith, A.J., et al. J. Acquir. Immune Defic. Syndr. 55(3):306-315(2010)
Background Reference 2:
Kojima, F., et al. Oncol. Rep. 23(4):927-931(2010)
Background Reference 3:
Szakal, D.N., et al. Virchows Arch. 456(3):245-250(2010)
Background Reference 4:
Buchert, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(6):2628-2633(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-552

List Size:
400 ul

List Price:

$495.00

Datasheet
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