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Western blot analysis in Jurkat cell line lysates (35ug/lane).
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Jurkat cell line lysates (35ug/lane).

CLDN22 Antibody

CATALOG NUMBER: 55-099

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This CLDN22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-117 amino acids from the Central region of human CLDN22.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
25 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CLDN22
Additional Names:
Claudin-22, CLDN22
Protein Accession Number:
Q8N7P3
Protein Gi Number:
62298548
Ncbi Gene Id Number:
53842
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the claudin family. Claudinsare integral membrane proteins and components of tight junctionstrands. Tight junction strands serve as a physical barrier toprevent solutes and water from passing freely through theparacellular space between epithelial or endothelial cell sheets,and also play critical roles in maintaining cell polarity andsignal transductions. This gene is intronless and overlaps the 3'UTR of the WWC2 gene (GeneID: 80014) on the opposite strand.
Background Reference 1:
Lal-Nag, M., et al. Genome Biol. 10 (8), 235 (2009) :
Background Reference 2:
Krause, G., et al. Biochim. Biophys. Acta 1778(3):631-645(2008)
Background Reference 3:
Hillier, L.W., et al. Nature 434(7034):724-731(2005)
Background Reference 4:
Katoh, M., et al. Int. J. Mol. Med. 11(6):683-689(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-099

List Size:
400 ul

List Price:

$495.00

Datasheet
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