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Western blot analysis in Hela cell line lysates (35ug/lane).
CLDN7 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Hela cell line lysates (35ug/lane).

CLDN7 Antibody

CATALOG NUMBER: 55-071

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CLDN7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 172-201 amino acids from the C-terminal region of human CLDN7.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
22 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CLDN7
Additional Names:
Claudin-7, CLDN-7, CLDN7, CEPTRL2, CPETRL2
Protein Accession Number:
O95471
Ncbi Gene Id Number:
1366
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the claudin family. Claudinsare integral membrane proteins and components of tight junctionstrands. Tight junction strands serve as a physical barrier toprevent solutes and water from passing freely through theparacellular space between epithelial or endothelial cell sheets,and also play critical roles in maintaining cell polarity andsignal transductions. Differential expression of this gene has beenobserved in different types of malignancies, including breastcancer, ovarian cancer, hepatocellular carcinomas, urinary tumors,prostate cancer, lung cancer, head and neck cancers, thyroidcarcinomas, etc.. Alternatively spliced transcript variantsencoding different isoforms have been found.
Background Reference 1:
Kojima, F., et al. Oncol. Rep. 23(4):927-931(2010)
Background Reference 2:
Rendon-Huerta, E., et al.Gastrointest Cancer 41(1):52-59(2010)
Background Reference 3:
Ouban, A., et al. Histol. Histopathol. 25(1):83-90(2010)
Background Reference 4:
Kaarteenaho, R., et al. Respir. Res. 11, 59 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-071

List Size:
400 ul

List Price:

$495.00

Datasheet
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