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Western blot analysis of lysates from GSDMB-C-6-his protein, using Cleaved Gasdermin B（C Terminal） Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 0.3s. | <b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b>
Western blot analysis of lysates from 293T GSDMB stable cells and 293T GSDMB stable cells transfected with GZMA using Cleaved Gasdermin B（C Terminal） Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s. | <b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b>
Western blot analysis of lysates from GSDMB-C-6-his protein, using Cleaved Gasdermin B（C Terminal） Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 0.3s. | WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Cleaved Gasdermin B（C Terminal） Rabbit Recombinant Antibody (82-926)

CATALOG NUMBER: 82-926

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
GSDMB-C-6-his, 293T GSDMB stable cells transfected with GZMA
Predicted Molecular Weight:
18kDa/45kDa/46kDa/47kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GSDMB
Additional Names:
GSDML; PP4052; GSDMB-1; PRO2521; Cleaved Gasdermin B（C Terminal）
Protein Accession Number:
Q8TAX9
Ncbi Gene Id Number:
55876
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the gasdermin-domain containing protein family. Other gasdermin-family genes are implicated in the regulation of apoptosis in epithelial cells, and are linked to cancer. Alternative splicing and the use of alternative promoters results in multiple transcript variants. Additional variants have been described, but they are candidates for nonsense-mediated mRNA decay (NMD) and are unlikely to be protein-coding.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-926

List Size:
100 uL

List Price:

$540.00

Datasheet
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