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Western blot analysis of lysates from NIH/3T3 cells using Cleaved PARP Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated by staurosporine(1 μM) for 3 hour. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.
Western blot analysis of lysates from NIH/3T3 cells using Cleaved PARP Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated by staurosporine(1 μM) for 3 hour. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.

Cleaved PARP (Asp214) Rabbit Recombinant Antibody (83-451)

CATALOG NUMBER: 83-451

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:1000 - 1:2000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
NIH/3T3
Predicted Molecular Weight:
113kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Parp1
Additional Names:
PARP; PPOL; ARTD1; Adprp; Adprt1; msPARP; parp-1; sPARP-1; 5830444G22Rik
Protein Accession Number:
Q921K2
Ncbi Gene Id Number:
11545
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enables NAD+ ADP-ribosyltransferase activity; chromatin binding activity; and protein ADP-ribosylase activity. Involved in negative regulation of telomere maintenance via telomere lengthening; positive regulation of single strand break repair; and voluntary musculoskeletal movement. Acts upstream of or within several processes, including DNA metabolic process; behavioral response to cocaine; and protein poly-ADP-ribosylation. Located in cytoplasm; nucleolus; and nucleoplasm. Part of protein-containing complex. Is expressed in several structures, including Peyer's patch; brain; gut; immune system; and retina. Human ortholog(s) of this gene implicated in several diseases, including arthritis; bacterial meningitis; neurodegenerative disease (multiple); stomach carcinoma; and type 2 diabetes mellitus. Orthologous to human PARP1 (poly(ADP-ribose) polymerase 1).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-451

List Size:
100 uL

List Price:

$477.00

Datasheet
Shipping Info

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