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Western blot analysis of lysates from wild type (WT) and Cleaved PARP1 p25 knockout (KO) cells using Cleaved PARP1 p25 Rabbit mAb at 1:10000 dilution incubated at room temperature for 1.5 hours. Wild type (WT) and PARP1 knockout (KO) HeLa cells were treated with Staurosporine(1 μM) at 37℃ for 6 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 1s.
Western blot analysis of lysates from Jurkat cells using Cleaved PARP1 p25 Rabbit mAb at 1:10000 dilution incubated at room temperature for 1.5 hours. Jurkat cells were treated with Etoposide (25 μM) at 37℃ for 5 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 1s.
Western blot analysis of lysates from NIH/3T3 cells using Cleaved PARP1 p25 Rabbit mAb at 1:10000 dilution incubated at room temperature for 1.5 hours. NIH/3T3 cells were treated with Staurosporine(1 μM) for 3 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.
Immunoprecipitation of Cleaved PARP1 p25 from 600 µg extracts of Jurkat cells was performed using 2 µg of Cleaved PARP1 p25 Rabbit mAb. Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Cleaved PARP1 p25 Rabbit mAb at a dilution of 1:1000.
Western blot analysis of lysates from wild type (WT) and Cleaved PARP1 p25 knockout (KO) cells using Cleaved PARP1 p25 Rabbit mAb at 1:10000 dilution incubated at room temperature for 1.5 hours. Wild type (WT) and PARP1 knockout (KO) HeLa cells were treated with Staurosporine(1 μM) at 37℃ for 6 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 1s.

Cleaved PARP1 p25 Rabbit Recombinant Antibody (81-527)

CATALOG NUMBER: 81-527

Clonality:
Monoclonal
Tested Applications:
ELISA, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-214 of human Cleaved PARP1 p25 (P09874).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP, WB
Application Note:
WB,1:10000 - 1:40000, IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Jurkat treated with Etoposide, NIH/3T3 treated with Staurosporine, HeLa treated with Staurosporine
Predicted Molecular Weight:
113 kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PARP1
Additional Names:
PARP; PARS; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; Poly-PARP; Cleaved PARP1 p25
Protein Accession Number:
P09874
Ncbi Gene Id Number:
142
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

81-527

List Size:
100 uL

List Price:

$540.00

Datasheet
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