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43-506 (2ug/ml) staining of U937 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-506 (2ug/ml) staining of U937 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CLIC1 / NCC27 Antibody

CATALOG NUMBER: 43-506

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-TEVHTDTNKIEE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 30kDa band observed in lysates of cell line U937 (calculated MW of 26.9kDa according to NP_001279.2). Recommended concentration: 1-3ug/ml.
Predicted Molecular Weight:
Approx 30kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CLIC1 / NCC27
Additional Names:
CLIC1, chloride intracellular channel 1, G6, NCC27, RNCC protein, chloride channel ABP, chloride intracellular channel protein 1, hRNCC, nuclear chloride ion channel 27, nuclear chloride ion channel protein, p64CLCP, regulatory nuclear chloride ion channel protein
Protein Accession Number:
NP_001279.2
Protein Gi Number:
14251209
Ncbi Gene Id Number:
1192
Background Reference 1:
Goodchild SC, Angstmann CN, Breit SN, Curmi PM, Brown LJ.Transmembrane extension and oligomerization of the CLIC1 chloride intracellular channel protein upon membrane interaction.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-506

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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