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43-364 (0.03ug/ml) staining of Mouse (A) and Rat (B) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-364 (0.03ug/ml) staining of Mouse (A) and Rat (B) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CLOCK Antibody

CATALOG NUMBER: 43-364

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Cow
Immunogen:
The immunogen for this antibody is: C-DTSTPPRQHLPAHEK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Mouse and Rat Skeletal Muscle lysates (calculated MW of 96.4kDa according to Mouse NP_031741.1 and 97.0kDa according to Rat NP_068628.1). Recommended concentration: 0.03-0.1ug/ml.
Specificity:
Reported variants represent identical protein: NP_004889.1, NP_001254772.1
Predicted Molecular Weight:
Approx 100kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CLOCK
Additional Names:
CLOCK, clock homolog (mouse), KAT13D, bHLHe8, circadian locomoter output cycles kaput protein, circadian locomoter output cycles protein kaput, class E basic helix-loop-helix protein 8
Protein Accession Number:
NP_004889.1
Protein Gi Number:
4758010
Ncbi Gene Id Number:
9575
Background Reference 1:
Williams SR, Zies D, Mullegama SV, Grotewiel MS, Elsea SH.Smith-Magenis syndrome results in disruption of CLOCK gene transcription and reveals an integral role for RAI1 in the maintenance of circadian rhythmicity.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-364

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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