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Western blot analysis of CLPH in human testis tissue lysate with CLPH antibody at 1 μg/mL.
Immunohistochemistry of CLPH in human testis tissue with CLPH antibody at 5 μg/mL.
Western blot analysis of CLPH in human testis tissue lysate with CLPH antibody at 1 μg/mL.
Trial Size

CLPH Antibody

CATALOG NUMBER: 5497

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (75%), Mouse: (70%)
Immunogen:
CLPH antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human CLPH.
The immunogen is located within the last 50 amino acids of CLPH.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
CLPH antibody can be used for detection of CLPH by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2:
Cat. No. 11-701 - Human Testis Tissue Slide
Purification:
CLPH Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CLPH Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CLPH antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CABS1
Additional Names:
CLPH Antibody: CLPH, C4orf35, NYD-SP26, Calcium-binding and spermatid-specific protein 1, Testis development protein NYD-SP26
Protein Accession Number:
NP_149113
Protein Gi Number:
90652863
Ncbi Gene Id Number:
85438
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CLPH Antibody: CLPH (Casein-Like phosphoprotein) is a recently discovered, evolutionarily conserved, testis-specific cytoplasmic protein expressed exclusively in postmeiotic germ cells in rat spermatogenesis. CLPH is a novel casein kinase 2-phosphorylated disordered and calcium-binding protein that displays strong variation in relative expression levels during spermatogenesis. It is frequently associated with the mitochondrial inner membrane during the last steps of spermatid differentiation and is involved in the extremely complex structural rearrangements occurring in haploid germ cells during spermiogenesis.
Background Reference 1:
Calvel P, Kervarrec C, Lavigne R, et al. CLPH, a novel casein kinase 2-phosphorylated disordered protein, is specifically associated with postmeiotic germ cells in rat spermatogenesis. J. Proteome Res.2009; 8:2953-65.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5497

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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