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45-406 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-406 (2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
45-406 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CLPP Antibody

CATALOG NUMBER: 45-406

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-EAAPAAEPVPAST
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 26kDa band observed in Human Heart (calculated MW of 30.2kDa according to NP_006003.1). Recommended for use at 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Liver shows cytoplasmic staining consistent with mitochondrai in hepatocytes. Recommended concentration, 2-4ug/ml.
Positive Control 1:
Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CLPP
Additional Names:
CLPP, ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog (E. coli), ATP-dependent proteolytic subunit E.coli homolog, ATP-dependent protease ClpAP (E. coli), proteolytic subunit, human, ClpP (caseinolytic protease, ATP-dependent, proteolytic subunit, E. coli) homolog, ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli), ATP-dependent protease ClpAP, proteolytic subunit, human, ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog, caseinolytic peptidase, ATP-dependent, proteolytic subunit
Protein Accession Number:
NP_006003.1
Protein Gi Number:
5174419
Ncbi Gene Id Number:
8192
Background Reference 1:
Bross P, Andresen BS, Knudsen I, Kruse TA, Gregersen N. Human ClpP protease: cDNA sequence, tissue-specific expression and chromosomal assignment of the gene. FEBS Lett. 1995 Dec 18;377(2):249-52.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-406

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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