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Western blot analysis of extracts of various cell lines, using CLUH antibody (13-454) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human placenta using CLUH antibody (13-454) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CLUH antibody (13-454) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using CLUH antibody (13-454) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using CLUH Polyclonal Antibody (13-454) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using CLUH Polyclonal Antibody (13-454) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CLUH antibody (13-454) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

CLUH Antibody

CATALOG NUMBER: 13-454

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1120-1309 of human CLUH (NP_056044.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:1000 - 1:4000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
K-562
Positive Control 3:
MCF7
Positive Control 4:
U-251MG
Positive Control 5:
LO2
Predicted Molecular Weight:
Observed: 180kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CLUH
Additional Names:
CLU1, clustered mitochondria protein homolog, clustered mitochondria (cluA/CLU1) homolog
Ncbi Gene Id Number:
23277
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-454

List Size:
100 uL

List Price:

$436.00

Datasheet
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