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46-749 (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-749 (0.3ug/ml) staining of Human Testes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-749 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
46-749 (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Clusterin Antibody

CATALOG NUMBER: 46-749

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-EKALQEYRRKSRAE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 38kDa band observed in Rat Brain and Human Testis lysates.(calculated MW of 51.4kDa according to Rat XP_006252156.1) The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources and the literature (Miyata et al, PMID: 11560857). The 38kDa band is thought to correspond to the alpha chain of APOJ. Recommended concentration: 0.1-1ug/ml. An additional band of unknown identity was also consistently observed at 28kDa in Rat Brain. This band was successfully blocked by incubation with the immunizing peptide.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Clu
Additional Names:
ApoJ, clusterin, Clu, AI893575, Cli, D14Ucla3, SP-40, Sgp-2, Sgp2, Sugp-2, Apolipoprotein J, complement lysis inhibitor, testosterone repressed prostate message
Protein Accession Number:
NP_038520.1
Protein Gi Number:
7304967
Ncbi Gene Id Number:
12759(mouse);24854(rat);
Background Reference 1:
Charnay Y, Imhof A, Vallet PG, Hakkoum D, Lathuilier A, Poku N, Aronow B. Kovari E, Bouras C, Giannakopoulos P. Clusterin expression during fetal and postnatal CNS development in mouse. Neuroscience. 2008 Jun 17.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-749

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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