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Clusterin Antibody [CLI-9]

CATALOG NUMBER: XA-1007

Clonality:
Monoclonal
Tested Applications:
ICC, IHC, IP, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Clusterin (monoclonal CLI-9) antibody was raised against human clusterin (TRPM-2).
Conjugate:
Unconjugated
Tested Applications:
ICC, IHC, IP, WB
Application Note:
Clusterin antibody can be used for detection of human clusterin by immunocytochemistry, immunohistochemistry (frozen sections), immunoprecipitation, and western blot (1:2,000). Method: : Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2,000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.
Purification:
Clusterin (monoclonal CLI-9) antibody was purified to ≥95% in SDS-PAGE.
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
PBS containing 0.02% sodium azide
Concentration:
batch dependent
Storage Conditions:
Clusterin antibody can be stored at 4°C, stable for one year.
Ncbi Gene Id Number:
1191
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background Reference 1:
Large-scale serial analysis of gene expression reveals genes differentially expressed in ovarian cancer: C. D. Hough, et al.; Cancer Res. 60, 6281 (2000).
Background Reference 2:
Characterization and cloning of androgen-repressed mRNAs from rat ventral prostate:J.G. Leger, et al.; BBRC 147, 196 (1987).
Background Reference 3:
Apolipoprotein J: structure and tissue distribution: H.V. De Silva, et al.; Biochemistry 29, 5380 (1990).
Background Reference 4:
Clusterin: the intriguing guises of a widely expressed glycoprotein: D.E. Jenne & J. Tschopp; Trends Biochem. Sci. 17, 154 (1992).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

XA-1007

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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