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HEK293 lysate (10ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with 42-759 (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with 42-759(1mg/ml) in Lane B. Primary
42-759 (1.5ug/ml) staining of paraffin embedded Human Testis (Mast cells). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-759 (1.5ug/ml) staining of paraffin embedded Human Tonsil (Mast cells). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
HEK293 lysate (10ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with 42-759 (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with 42-759(1mg/ml) in Lane B. Primary

CMA1 Antibody

CATALOG NUMBER: 42-759

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-QFRHPKYNTSTLHHD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing full-length Human CMA1 (myc and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.5-1ug/ml.Immunohistochemistry:Paraffin embedded Human Testis and Tonsil (Mast cells). Recommended concentration: 1.5ug/ml.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.
Positive Control 1:
Transfected HEK293
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CMA1
Additional Names:
CMA1, chymase 1, mast cell, CYH, MCT1, MGC119890, MGC119891, chymase 1 preproprotein transcript E, chymase 1 preproprotein transcript I, chymase, heart, chymase, mast cell, mast cell protease I
Protein Accession Number:
NP_001827.1
Protein Gi Number:
4502907
Ncbi Gene Id Number:
1215

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-759

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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