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Western blot analysis of extracts of various cell lines, using CMPK1 antibody (22-313) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of MCF7 cells using CMPK1 antibody (22-313). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CMPK1 antibody (22-313) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

CMPK1 Antibody

CATALOG NUMBER: 22-313

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 69-228 of human CMPK1 (NP_057392.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
293T
Positive Control 2:
HL-60
Positive Control 3:
HT-29
Positive Control 4:
HeLa
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse kidney
Predicted Molecular Weight:
Observed: 22kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CMPK1
Additional Names:
CMPK1, CMPK, Deoxycytidylate kinase, Cytidine monophosphate kinase, RP11-511I2.1, UMP-CMPK, UMP/CMP kinase, Uridine monophosphate kinase, UCK, UMK, UMP-CMP kinase, UMP/CMPK, CMK, Cytidylate kinase, UMPK
Ncbi Gene Id Number:
51727
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes one of the enzymes required for cellular nucleic acid biosynthesis. This enzyme catalyzes the transfer of a phosphate group from ATP to CMP, UMP, or dCMP, to form the corresponding diphosphate nucleotide. Alternate splicing results in both coding and non-coding transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-313

List Size:
100 uL

List Price:

$436.00

Datasheet
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