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42-116 (0.03ug/ml) staining of HepG2 lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-116 (2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-116 (0.03ug/ml) staining of HepG2 lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

CNN2 Antibody

CATALOG NUMBER: 42-116

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DPGEVPEYPPYYQEE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in lysates of cell line HepG2 (calculated MW of 33.7kDa according to NP_004359.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 2.5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_004359.1; NP_958434.1), but it is not expected to cross-react with CCN3.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CNN2
Additional Names:
CNN2, calponin 2, calponin H2, smooth muscle, neutral calponin
Protein Accession Number:
NP_004359.1,NP_958434.1
Protein Gi Number:
4758018
Ncbi Gene Id Number:
1265
Background Reference 1:
Masuda H, Tanaka K, Takagi M, Ohgami K, Sakamaki T, Shibata N, Takahashi K, Molecular cloning and characterization of human non-smooth muscle calponin. Journal of biochemistry 1996 Aug 120 (2): 415-24.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-116

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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