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Western blot analysis of CNOT4 in A549 lysate with CNOT4 antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of CNOT4 in A549 lysate with CNOT4 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

CNOT4 Antibody

CATALOG NUMBER: 4813

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
CNOT4 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of the human CNOT4.
The immunogen is located within amino acids 70 - 120 of CNOT4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
CNOT4 antibody can be used for detection of CNOT4 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Purification:
CNOT4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CNOT4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CNOT4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CNOT4
Additional Names:
CNOT4 Antibody: NOT4, NOT4H, CLONE243, NOT4, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4
Protein Accession Number:
NP_037448
Protein Gi Number:
56550057
Ncbi Gene Id Number:
4850
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CNOT4 Antibody: CNOT4 is a component of the CCR4-NOT transcription complex, a complex that is implicated in the repression of RNA polymerase II transcription. In the CCR4-NOT complex, CNOT4 acts as an E3 ubiquitin-protein ligase and interacts with a subset of E2 ubiquitin-conjugating enzymes through a unique C4C4 RING domain. This E3 ligase activity was shown to be dependent on the selective and specific interaction with the ubiquitin conjugating enzyme UbcH5B. In yeast, mutations in CNOT4 that prevented its interaction with the UbcH5B homolog UBC4 caused increased sensitivity to hydroxyurea, heat shock, and hygromycin B, suggesting that CNOT4 and UbcH5B are involved in stress response in vivo. Multiple isoforms of CNOT4 are known to exist.
Background Reference 1:
Albert TK, Hanzawa H, Legtenberg IA, et al. Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex. EMBO J.2002; 21:355-64.
Background Reference 2:
Winkler GS, Albert TK, Dominguez C, et al. An altered specificity ubiquitin-conjugating enzyme/ubiquitin-protein ligase pair. J. Mol. Biol.2004; 337:157-65.
Background Reference 3:
Mulder KW, Inagaki A, Cameroni E, et al. Modulation of Ubc4p/Ubc5p-mediated stress responses by the RING-finger-dependent ubiquitin-protein ligase Not4p in Saccharomyces cerevisiae. Genetics2007; 176:181-92.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4813

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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