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Western blot analysis of extracts of NIH3T3 cells, using CNR1 antibody (15-560) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human lung cancer using CNR1 antibody (15-560) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach cancer using CNR1 antibody (15-560) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CNR1 antibody (15-560) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using CNR1 antibody (15-560) at dilution of 1:150 (40x lens).
Western blot analysis of extracts of NIH3T3 cells, using CNR1 antibody (15-560) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.

CNR1 Antibody

CATALOG NUMBER: 15-560

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human CNR1 (NP_001153731.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
NIH/3T3
Predicted Molecular Weight:
Observed: 53kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CNR1
Additional Names:
central cannabinoid receptor, cannabinoid receptor 1, CNR, CB1R, CB1K5, CB1A, CB1, CB-R, CANN6, cannabinoid receptor 1 (brain), CNR1
Ncbi Gene Id Number:
1268
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-560

List Size:
100 uL

List Price:

$436.00

Datasheet
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